Methacrylic polymer B2 Supplier reports that volume changes due to ionic strength or pH are negligible.Ĥ3 F2 Value is provided in the Tosoh website. SP Sepharose Big Beads 02 GE Life Sciences (former Amersham biosciences) Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. Streamline SP XL 02 GE Life Sciences (former Amersham biosciences)Ģ00 (100-300) >140 E1 No data about the binding conditions. Streamline SP 02 GE Life Sciences (former Amersham biosciences)Īgarose, 6% cross-linked, quartz core, dextran surface extender B1 Supplier does not provide data about volume changes due to ionic strength or pH. >60 E1 No data about the binding conditions. Jensen, Journal of Chromatography A, 908, 2001, 149-161.Īgarose, 6% cross-inked, quartz core B1 Supplier does not provide data about volume changes due to ionic strength or pH. (human IgG) E Lysozyme: pI ~11, MW = 14.5 kDa Human IgG: pI~7.4-8.6, MW = 150 kDa Bovine COHb: MW = 69 kDa Ribonuclease: pI~9.6, MW = 14 kDA Insulin: pI~5.3, MW = 6kDa.Īgarose,Ĕ% cross-linked, hardened B2 Supplier reports that volume changes due to ionic strength or pH are negligible.ĥ0 E1 No data about the binding conditions.ĩ F1 By analogy with data for Q-Cellthru Bigbeads Plus: A. *See main Ion-Exchange Chromatography page for detailed description. We develop, manufacture and sell chromatography detectors and detector/gradient pump systems for FPLC, LPLC and MPLC. REACH Devices does not manufacture, does not distribute, and does not promote any ion-exchange resins. List of Commercially Available Strong Cation Exchangers * The multimodal property of the ligands in the core ensures strong binding with most impurities over a wide range of pH and salt concentrations.Protein Purification by Ion-Exchange Chromatography The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of the various contaminants small enough to enter into the core. Ensuring straightforward optimization and scale-up.Enabling significantly higher sample load.Relative to size-exclusion chromatography, Capto Core 700 provides the advantages of high purity, improved process economy, and increased productivity by: The pore size of the beads gives a restricted access to the ligand-activated core and provides a cut-off for molecules larger than Mr 700 000. The core bead design of this medium gives dualfunctional properties that make it possible to combine size separation with binding chromatography. The Capto Core 700 beads consist of a ligand-activated core and inert shell. The multimodal property of the ligand in the core ensures strong binding with most impurities over a wide range of pH and salt concentration. The performance of the medium can be compared with gel filtration chromatography, but Capto Core 700 offers improved productivity and process economy. Capto Core 700 is a multimodal chromatography medium that is designed for flowthrough purification of large proteins or viruses in vaccine processes.
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